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anti kv1 5  (Alomone Labs)


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    Structured Review

    Alomone Labs anti kv1 5
    Anti Kv1 5, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kv1+5/Anti-KV1%2E5+(KCNA5)+Antibody/pm41707437-92-11-14
    Average 95 stars, based on 49 article reviews
    anti kv1 5 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Incubation:

    Article Title: SNAP25-dependent membrane trafficking of the Kv1.5 channel regulates the onset of atrial fibrillation.
    Article Snippet: .. After quantification, protein lysates (same amounts in each group) were incubated for 2 hours at 4 °C with primary antibodies against the SNAP25 (Abcam), the Kv1.5 (Alomone), or isotype control immunoglobulin G (Sigma, USA) at a concentration of 1:50. .. Then, 50 μLDynabeads (Invitrogen, USA)was added to each tube, and the mixtures were incubated for another 2 hours at 4 °C.

    Article Title: Short-chain fatty acid butyrate against TMAO activating endoplasmic-reticulum stress and PERK/IRE1-axis with reducing atrial arrhythmia
    Article Snippet: .. Then, the blots were incubated with primary antibodies against phospho-PERK (pPERK; #3179, Cell signaling), PERK (#3192, Cell signaling), phospho-IRE1α (pIRE1α; NB100-2323, Novus), IRE1α (#3294, Cell signaling Technology), phospho-NF-κB (pNF-κB; #3033, Cell Signaling Technology), NF-κB (#8242, Cell Signaling Technology), phospho-IP3R (pIP3R; #3760, Cell signaling Technology), IP3R (#8568, Cell signaling Technology), NCX (MA3-926, Thermo Fisher Scientific), Kv1.5 (APC-004, Alomone Lab), and β-actin (sc-47778, Santa Cruz). ..

    Article Title: SNAP25-dependent membrane trafficking of the Kv1.5 channel regulates the onset of atrial fibrillation
    Article Snippet: .. After quantification, protein lysates (same amounts in each group) were incubated for 2 hours at 4 °C with primary antibodies against the SNAP25 (Abcam), the Kv1.5 (Alomone), or isotype control immunoglobulin G (Sigma, USA) at a concentration of 1:50. .. Then, 50 μL Dynabeads (Invitrogen, USA) was added to each tube, and the mixtures were incubated for another 2 hours at 4 °C.

    Control:

    Article Title: SNAP25-dependent membrane trafficking of the Kv1.5 channel regulates the onset of atrial fibrillation.
    Article Snippet: .. After quantification, protein lysates (same amounts in each group) were incubated for 2 hours at 4 °C with primary antibodies against the SNAP25 (Abcam), the Kv1.5 (Alomone), or isotype control immunoglobulin G (Sigma, USA) at a concentration of 1:50. .. Then, 50 μLDynabeads (Invitrogen, USA)was added to each tube, and the mixtures were incubated for another 2 hours at 4 °C.

    Article Title: SNAP25-dependent membrane trafficking of the Kv1.5 channel regulates the onset of atrial fibrillation
    Article Snippet: .. After quantification, protein lysates (same amounts in each group) were incubated for 2 hours at 4 °C with primary antibodies against the SNAP25 (Abcam), the Kv1.5 (Alomone), or isotype control immunoglobulin G (Sigma, USA) at a concentration of 1:50. .. Then, 50 μL Dynabeads (Invitrogen, USA) was added to each tube, and the mixtures were incubated for another 2 hours at 4 °C.

    Concentration Assay:

    Article Title: SNAP25-dependent membrane trafficking of the Kv1.5 channel regulates the onset of atrial fibrillation.
    Article Snippet: .. After quantification, protein lysates (same amounts in each group) were incubated for 2 hours at 4 °C with primary antibodies against the SNAP25 (Abcam), the Kv1.5 (Alomone), or isotype control immunoglobulin G (Sigma, USA) at a concentration of 1:50. .. Then, 50 μLDynabeads (Invitrogen, USA)was added to each tube, and the mixtures were incubated for another 2 hours at 4 °C.

    Article Title: SNAP25-dependent membrane trafficking of the Kv1.5 channel regulates the onset of atrial fibrillation
    Article Snippet: .. After quantification, protein lysates (same amounts in each group) were incubated for 2 hours at 4 °C with primary antibodies against the SNAP25 (Abcam), the Kv1.5 (Alomone), or isotype control immunoglobulin G (Sigma, USA) at a concentration of 1:50. .. Then, 50 μL Dynabeads (Invitrogen, USA) was added to each tube, and the mixtures were incubated for another 2 hours at 4 °C.

    Blocking Assay:

    Article Title: Association of the chemerin-CMKLR1 with atrial potassium current dysregulation and atrial fibrillation in obese mice.
    Article Snippet: After transfer, membranes were blocked with 5% skim milk (Solarbio, China) in TBST (Solarbio, China) for 2 h at room temperature. .. Primary antibodies diluted in blocking buffer were applied overnight at 4°C as follows: resistin (1:1,000; Abcam, USA), chemerin (1:1,000; Abcam, USA), leptin (1:1,000; Abcam, USA), CMKLR1 (1:1,000; InvitrogenTM, USA), Kv4.3 (1:1,000; Alomone Labs, Israel), Kv4.2 (1:1,000; Alomone Labs, Israel), KChIP2 (1:1,000; Alomone Labs, Israel), Kv1.5 (1:1,000; Alomone Labs, Israel), Kir2.1 (1:1,000; Alomone Labs, Israel), β-Tubulin (1:1,000; Abcam, USA). .. Membranes were washed three times with TBST (10 min each) and then incubated for1 h at room temperature with HRP-conjugated goat anti-rabbit IgG (1:10,000; Solarbio, China) for detection of rabbit primary antibodies.

    Article Title: Association of the chemerin-CMKLR1 with atrial potassium current dysregulation and atrial fibrillation in obese mice
    Article Snippet: After transfer, membranes were blocked with 5% skim milk (Solarbio, China) in TBST (Solarbio, China) for 2 h at room temperature. .. Primary antibodies diluted in blocking buffer were applied overnight at 4°C as follows: resistin (1:1,000; Abcam, USA), chemerin (1:1,000; Abcam, USA), leptin (1:1,000; Abcam, USA), CMKLR1 (1:1,000; InvitrogenTM, USA), Kv4.3 (1:1,000; Alomone Labs, Israel), Kv4.2 (1:1,000; Alomone Labs, Israel), KChIP2 (1:1,000; Alomone Labs, Israel), Kv1.5 (1:1,000; Alomone Labs, Israel), Kir2.1 (1:1,000; Alomone Labs, Israel), β-Tubulin (1:1,000; Abcam, USA). .. Membranes were washed three times with TBST (10 min each) and then incubated for1 h at room temperature with HRP-conjugated goat anti-rabbit IgG (1:10,000; Solarbio, China) for detection of rabbit primary antibodies.

    Article Title: Decreased PLK2 promotes atrial fibrillation in diabetic mice through Nrf2/HO-1 pathway.
    Article Snippet: Diabetes mellitus (DM) is a chronic and systemic metabolic disease with increasing prevalence worldwide, and type 2 diabetes mellitus (T2DM), characterized by insulin resistance, accounts for approximately 90% of diabetes cases [1].. Atrial fibrillation (AF) is the most common sustained arrhythmia in the clinical practice and carries a high burden of mortality and morbidity [2].. It has been suggested that the incidence of AF is substantially increased in T2DM, which may be attributed to the atrial electrical remodeling and structural remodeling caused by DM [3].



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